3-硝基酪氨酸(3-NT)酶聯(lián)免疫吸附檢測試劑盒-ELK科鹿生物-ELISA試劑盒、抗體、蛋白、分子

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3-硝基酪氨酸(3-NT)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK7866

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview 文獻(xiàn)(3)

Product name:	3-NT(3-Nitrotyrosine) ELISA Kit
Reactivity:	General
Alternative Names:	3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine
Assay Type:	Competitive Inhibition
Sensitivity:	1.4 ng/mL
Standard:	300 ng/mL
Detection Range:	4.69-300 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Metabolic pathway;Infection immunity;Rheumatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.

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標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
300.00	0.217
150.00	0.467
75.00	0.793
37.50	0.967
18.75	1.236
9.38	1.596
4.69	1.861
0.00	2.265

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 3-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 3-NT in samples.

Matrix	Recovery range	Average
serum(n=5)	92-107%	99%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	78-92%	84%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.