小鼠瓜氨酸組蛋白H3(Cit H3)微量上樣酶聯免疫吸附檢測試劑盒-ELK科鹿生物-ELISA試劑盒、抗體、蛋白、分子

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小鼠瓜氨酸組蛋白H3(Cit H3)微量上樣酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK11035MS

規格：	價格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下載說明 ①

Overview

Product name:	Mouse Cit H3(Citrullinated Histone H3) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; ; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l;
Assay Type:	Sandwich
Sensitivity:	15.1 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Epigenetics and Nuclear Signaling
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Cit H3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Cit H3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Cit H3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Cit H3 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.230	2.145
1000.00	1.602	1.517
500.00	1.209	1.124
250.00	0.944	0.859
125.00	0.489	0.404
62.50	0.342	0.257
31.25	0.224	0.139
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Cit H3 and the recovery rates were calculated by comparing the measured value to the expected amount of Cit H3 in samples.

Matrix	Recovery range	Average
serum(n=5)	91-109%	100%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	90-101%	96%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cit H3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.