人透明質酸(HA)酶聯免疫吸附檢測試劑盒-ELK科鹿生物-ELISA試劑盒、抗體、蛋白、分子

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人透明質酸(HA)酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK10906

規格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview 文獻(1)

Product name:	Human HA(Hyaluronic Acid) ELISA Kit
Reactivity:	Human
Alternative Names:	Hyaluronan; Hyaluronate
Assay Type:	Competitive Inhibition
Sensitivity:	4.84 ng/mL
Standard:	1000 ng/mL
Detection Range:	15.63-1000 ng/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Tumor immunity;Hepatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HA in the samples is then determined by comparing the OD of the samples to the standard curve.

Antioxidants

Natural Bioactive Peptides from Tree Peony Flowers: Multifunctional Effects on Skin Antioxidation, Wrinkle Reduction, Moisturization, and Melanin Inhibition

日期：2026-03-11 IF：6.6

標準曲線

Concentration (ng/mL)	OD	Corrected OD
1000.00	0.182
500.00	0.397
250.00	0.583
125.00	0.993
62.50	1.139
31.25	1.596
15.63	1.877
0.00	2.211

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HA and the recovery rates were calculated by comparing the measured value to the expected amount of HA in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	83-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.