大鼠骨骼肌快肌肌鈣蛋白I(TNNI2)微量上樣酶聯免疫吸附檢測試劑盒-ELK科鹿生物-ELISA試劑盒、抗體、蛋白、分子

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大鼠骨骼肌快肌肌鈣蛋白I(TNNI2)微量上樣酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK10739MS

規格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Rat TNNI2(Troponin I Type 2, Fast Skeletal) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	AMCD2B; DA2B; FSSV; Troponin I Fast Twitch 2; Troponin I, fast-twitch isoform
Assay Type:	Sandwich
Sensitivity:	23.1 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Cardiovascular biology;
Uniprot ID:	P27768
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNNI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNNI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNNI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNNI2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.034	1.937
2500.00	1.665	1.568
1250.00	1.200	1.103
625.00	0.872	0.775
312.50	0.584	0.487
156.25	0.324	0.227
78.13	0.207	0.110
0.00	0.097	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TNNI2 and the recovery rates were calculated by comparing the measured value to the expected amount of TNNI2 in samples.

Matrix	Recovery range	Average
serum(n=5)	91-103%	97%
EDTA plasma(n=5)	88-119%	104%
Heparin plasma(n=5)	82-116%	99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNNI2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.