小鼠氨基端前心鈉肽(NT-ProANP)微量上樣酶聯免疫吸附檢測試劑盒-ELK科鹿生物-ELISA試劑盒、抗體、蛋白、分子

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小鼠氨基端前心鈉肽(NT-ProANP)微量上樣酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK10410MS

規格：	價格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下載說明 ①

Overview

Product name:	Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	NT-Pro-ANP; N-ANP
Assay Type:	Sandwich
Sensitivity:	0.31 ng/mL
Standard:	50 ng/mL
Detection Range:	0.79-50 ng/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
50.00	1.980	1.889
25.00	1.610	1.519
12.50	1.111	1.020
6.25	0.747	0.656
3.13	0.606	0.515
1.56	0.238	0.147
0.78	0.170	0.079
0.00	0.091	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NT-ProANP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProANP in samples.

Matrix	Recovery range	Average
serum(n=5)	84-104%	94%
EDTA plasma(n=5)	82-98%	90%
Heparin plasma(n=5)	92-119%	106%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.