高敏型人幾丁質酶3樣蛋白1(CHI3L1)酶聯免疫吸附檢測試劑盒-ELK科鹿生物-ELISA試劑盒、抗體、蛋白、分子

產品中心

高敏型人幾丁質酶3樣蛋白1(CHI3L1)酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK10135HS

規格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	High Sensitivity Human CHI3L1(Chitinase-3-Like Protein 1) ELISA Kit
Reactivity:	Human
Alternative Names:	YKL-40; Chondrocyte protein 40
Assay Type:	Sandwich
Sensitivity:	45.5 pg/mL
Standard:	3000 pg/mL
Detection Range:	46.88-3000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	3.5h
Research Area:	Metabolic pathway;
Uniprot ID:	1:20-1:80
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CHI3L1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CHI3L1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CHI3L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CHI3L1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
3000.00	2.328	2.239
1500.00	1.731	1.642
750.00	1.251	1.162
375.00	0.892	0.803
187.50	0.548	0.459
93.75	0.355	0.266
46.88	0.227	0.138
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CHI3L1 and the recovery rates were calculated by comparing the measured value to the expected amount of CHI3L1 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-101%	95%
EDTA plasma(n=5)	91-104%	98%
Heparin plasma(n=5)	86-95%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CHI3L1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.