小鼠層粘連蛋白γ2(LAMC2)微量上樣酶聯(lián)免疫吸附檢測(cè)試劑盒-ELK科鹿生物-ELISA試劑盒、抗體、蛋白、分子

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小鼠層粘連蛋白γ2(LAMC2)微量上樣酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK0051MS

規(guī)格：	價(jià)格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下載說(shuō)明 ①

Overview

Product name:	Mouse LAMC2(Laminin Gamma 2) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	B2T; EBR2; EBR2A; LAMB2T; LAMNB2; Kalinin/nicein/epiligrin 100 kDa subunit; Cell-scattering factor 140 kDa subunit; Ladsin 140 kDa; Large adhesive scatter factor 140 kDa
Assay Type:	Sandwich
Sensitivity:	7 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Developmental science;
Uniprot ID:	Q61092
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LAMC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LAMC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LAMC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LAMC2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.140	2.052
500.00	1.605	1.517
250.00	1.157	1.069
125.00	0.889	0.801
62.50	0.493	0.405
31.25	0.240	0.152
15.63	0.230	0.142
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LAMC2 and the recovery rates were calculated by comparing the measured value to the expected amount of LAMC2 in samples.

Matrix	Recovery range	Average
serum(n=5)	91-118%	105%
EDTA plasma(n=5)	89-99%	94%
Heparin plasma(n=5)	93-109%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LAMC2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.